Regulatory

Part:BBa_K2796052:Design

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-05)


HRE(Hypoxia response elements)-miniCMV

    The part we designed and plasmid map are shown below:
    2018 LZU-CHINA 52experimental design1.png

    Agarose gel electrophoresis

    We plan to use phRE to adjust the expression of EX.We designed a promoter with five HRE repeats and tested its function with copGFP.The results of agarose gel electrophoresis for phre-minicmv are shown below.

    2018 LZU-CHINA 52experimental Gel.png


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal XhoI site found at 5
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]


    Design Notes

    we repeat HRE for 5 times to increase the binding efficiency of HIF-1.


    Source

    NCBI

    ===References===