Regulatory
Part:BBa_K2796052:Design
Designed by: Shuangshuang Pu Group: iGEM18_LZU-CHINA (2018-10-05)
HRE(Hypoxia response elements)-miniCMV
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The part we designed and plasmid map are shown below:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 5
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Agarose gel electrophoresis
We plan to use phRE to adjust the expression of EX.We designed a promoter with five HRE repeats and tested its function with copGFP.The results of agarose gel electrophoresis for phre-minicmv are shown below.
Assembly Compatibility:
Design Notes
we repeat HRE for 5 times to increase the binding efficiency of HIF-1.
Source
NCBI
===References===